2025 BBRL Biosafety Crossword

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Across
  1. 2. Eye __________ should be worn when working with chemicals.
  2. 6. A _____ container can be filled only ¾ before it must be sealed for disposal. All contaminated glass must be placed in this container.
  3. 7. Wipe all work surfaces with an appropriate disinfectant ( _______________ spray or Sani-wipes) after experiments. In case of a spill, stop work immediately and follow the spill clean-up procedure posted on the back of the lab door.
  4. 11. Any accident, injury, illness, or over exposure must be ____________ reported to your principal investigator.
  5. 13. After working with ________, the work surface should be cleaned immediately with Sani-Wipes and/or 70% isopropyl alcohol.
  6. 14. Remember to wash your _____ after handling animals, after removing PPE, and before exiting the lab.
  7. 15. To dispose of unwanted chemicals, label them “________ Material” and contact Brooke. She will arrange EHS disposal.
  8. 16. Liquid waste must be decontaminated for at least ______ minutes before disposal.
  9. 17. Most laboratory-acquired infections have resulted from inhalation of aerosols, splashes or sprays, or ______ sticks. It is good practice to look for potential exposures via ingestion, inoculation, inhalation, and contamination of skin and mucous membranes and attempt to identify safer alternatives and risk mitigation strategies.
  10. 19. Bacteria should be enclosed in capped containers while vortexing, shaking, centrifugation, and sonication to avoid generating ________.
  11. 20. All bacterial isolates are kept ______ and only thawed as needed per experimental protocol.
  12. 21. Never mix bleach with ammonium chemicals (____-Wipes or Sani-Plex 128M).
Down
  1. 1. After agitation, containers should be opened in a ________ cabinet to prevent exposure to aerosols.
  2. 3. Minimal PPE in the microbiology lab includes ___ coat or gown, gloves, closed-toe shoes, and long pants.
  3. 4. Bleach mixes with ammonia compounds to produce very toxic __________ gas. Bleach is corrosive to plastics and metals. Surfaces should be wiped down with alcohol after bleach disinfection to prevent corrosion.
  4. 5. All skin defects (cuts, abrasions, ulcers, dermatitis, etc.) should be _______ with a bandage.
  5. 8. Dilutions of sodium hypochlorite (______) should be made twice per week.
  6. 9. No ______, chewing gum, drinking, smoking, handling contact lenses, or applying cosmetics in the lab at any time.
  7. 10. Eye __________ should be worn when liquid splashes could occur.
  8. 11. Bacterial samples should be carefully carried from an in-lab freezer to your workspace, where they are used and ____________ returned.
  9. 12. A ____ should also be worn when you are within 6 feet of another person who is working with bacteria to prevent sample contamination.
  10. 18. ______ should be immediately changed after direct contact with bacteria of any kind to protect your hands.