Biotechnology Crossword

3. Technique used to identify a particular DNA species. DNA is separated by electrophoresis and transferred to nitrocellulose
4. cells that have the potential to become any type of cell
6. Specific DNA sequence recognized by a restriction enzyme
8. Technique that uses the enzyme reverse transcriptase to make DNA from RNA followed by amplification of the DNA
10. use of an electrical current to create small holes in a cell membrane
12. variation in the nucleotide of a single base pair of a genome that is found in approximately 1% of the population
13. differentiated cells that can be coaxed in the lab to rediscover themselves
15. technique that identifies a particular mRNA sequence
16. Bacterial scissors or enzymes that recognize specific DNA sequences and cut the DNA at those sites
17. food that is, whole or in part, produced with crops that have had modifications made to their genetic material
19. DNA sequence and the cloning vector into which it has been inserted

1. pieces of DNA formed as the result of being cut by a restriction enzyme
2. technique used to amplify small amounts of DNA
5. removal of the nucleus of one cell and insertion into an egg cell where the nucleus has been removed
7. enzyme that acts like a glue connecting the phosphate backbone of a piece of DNA inserted into a cloning vector
9. field that generates useful products by manipulating living systems and their components
11. process of deciding what traits should be expressed in the next generation
14. small piece of DNA, maintained separately from the chromosome
16. Technique that uses the enzyme reverse transcriptase to make DNA from RNA followed by amplification of the DNA
18. circular piece of DNA that has been souped-up for the easy insertion and expression of a piece of DNA