Downstream Bioprocessing Crossword

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Across
  1. 2. Protein purification instrument used in this lab
  2. 4. All waste must be sterilized with bleach before disposal
  3. 6. Type of antibody produced in mammalian cell culture
  4. 9. Antibiotic used to select modified E. coli cells
  5. 10. Measure of how well two proteins are separated
  6. 13. Membrane property defining molecular weight cutoff
  7. 14. Cell lysis method using ultrasonic vibrations
  8. 20. Common microbial host for recombinant protein expression
  9. 21. Solid material collected at the bottom after centrifugation
  10. 22. Molecule used to elute His-tagged proteins
  11. 23. Mechanical disruption method using glass beads
  12. 25. Vessel where cells are cultivated for protein expression
  13. 26. Chromatography that separates using hydrophobicity
  14. 28. Wavelength used to detect protein concentration generally
  15. 29. Chromatography that separates by protein size
  16. 32. Unique property of GFP used for measurement
  17. 33. First step of protein expression: DNA to RNA
  18. 34. Tangential flow filtration, concentrates or diafilters proteins
  19. 35. Type of membrane cartridge used for filtration
  20. 37. Protein expressed in this lab, naturally from jellyfish
  21. 38. Culture mode where nutrients are added gradually
  22. 39. Method to separate proteins by size with membranes
  23. 40. Optical density measurement of cell density
Down
  1. 1. General term for processing steps after cell growth
  2. 3. Second step of protein expression: RNA to protein
  3. 5. Chromatography type that uses a His tag binding to nickel
  4. 7. Main protein purification method
  5. 8. Equation relating absorbance to concentration
  6. 11. Classic therapeutic protein produced by biotech industry
  7. 12. Fraction of protein product successfully collected
  8. 15. Chromatography method separating by net protein charge
  9. 16. Liquid above the pellet after centrifugation
  10. 17. Process of removing bound proteins from a column
  11. 18. Step needed to release proteins from inside cells
  12. 19. Method to determine living cells by plating colonies
  13. 24. Specific absorbance peak for GFP detection
  14. 27. Chemical that triggers recombinant gene expression
  15. 30. Ratio of cells produced to nutrients consumed
  16. 31. Separation method using rotational force
  17. 36. Culture mode where all nutrients are added at the start