Electrophoresis

2. PH, Proteins can be separated using electrophoresis based on?
6. The direction of fluid is towards?
8. The gel commonly used in PAGE to separate proteins
13. a buffer not to be used for proteins
15. the parameter that determines the degree of ionization of organic compounds
16. The overall speed of protein migration in native PAGE depends on size and this property
19. (Sodium Dodecyl Sulfate),A method of PAGE that denatures proteins, unlike native PAGE
21. Migration of charged particles in medium requires?
23. The Fragment of DNA with a known sequence used as a standard in electrophoresis
25. this fluorescent dye binds between the stacked base pairs of DNA, making it visible under ultraviolet light

1. SIEVING, poor medium causes
3. (Coomassie Blue),Stain used to visualize proteins after native PAGE
4. GRADIENT, Gradient formed in isoelectric focusing to separate protein
5. POTENTIAL, Factor influencing electroendosmosis flow?
7. (Tris-Borate-EDTA buffer),The buffer system often used to maintain protein structure during native PAGE
9. proteins if globular, then surface area is?
10. the typical shape of double stranded DNA molecules, which affects how they move through gel pores
11. MERCAPTOETHANOL, Reducing agent that breaks disulfide bonds in proteins
12. Type of material exhibiting electroendosmosis
14. DYE, Dye used in electrophoresis to monitor sample migration
17. This enzyme is commonly used to cleave DNA into fragments for electrophoresis
18. DNA, suppose there are 3 types of DNA- ssDNA ,dsDNA and ds plasmid and you are to carry out electrophoresis, which of these will have faster electrophoretic mobility?
20. Used to form loading wells in a gel, removed after the gel has set
22. GEL, Gel component with lower acrylamide concentration for protein separation
24. GEL, The gel layer responsible for separating protein by size