Flow Cytometry

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Across
  1. 7. CD8 T cells will fluoresce this color when tested for CD3 and CD4
  2. 8. CD__________ is a B-lymphocyte cell marker
  3. 10. MoABs are attached to __________ for fluorescent tagging
  4. 14. CD__________ is a panleukocytic marker
  5. 15. PBS stands for __________ saline
  6. 19. cells exit the flow cell in a __________ formation
  7. 21. kappa and lambda are two types of __________ that make up antibodies
Down
  1. 1. this is used to lyse red cells while maintaining WBC integrity
  2. 2. CD stands for cluster __________
  3. 3. in this type of leukemia, B cells express CD5, CD22, and are dimly CD20 and CD23 (coexpression) positive
  4. 4. bone marrow specimens containing __________ need to be filtered
  5. 5. this type of scatter provides information about a cell's granular content and complexity
  6. 6. a boundary set up around a subset of data points for analysis or to exclude other events from the analysis
  7. 9. the emitted __________ of a cell during flow represents the extrinsic cell characteristics
  8. 11. specimens are incubated with this type of antibody for analysis
  9. 12. CD4 T cells will fluoresce orange and __________ when tested for CD3 and CD4
  10. 13. CD3 marks all __________ T cells
  11. 16. this type of scatter provides information about cell size
  12. 17. __________ cells are preferable over dead cells in flow analysis
  13. 18. this measurement taken during flow reflects the intrinsic characteristics of the cell
  14. 20. the combination of CD11c, CD25, and CD103 positive lymphocytes is an indicator of this chronic leukemia