game_1

1234567891011121314151617181920212223
Across
  1. 4. Factor influencing electroendosmosis flow?
  2. 7. The overall speed of protein migration in native PAGE depends on size and this property
  3. 9. Dye used in electrophoresis to monitor sample migration
  4. 11. the typical shape of double stranded DNA molecules, which affects how they move through gel pores
  5. 14. Stain used to visualize proteins after native PAGE
  6. 15. The direction of fluid is towards?
  7. 16. The gel layer responsible for separating protein by size
  8. 17. this fluorescent dye binds between the stacked base pairs of DNA, making it visible under ultraviolet light
  9. 19. a buffer not to be used for proteins
  10. 21. A method of PAGE that denatures proteins, unlike native PAGE
  11. 22. proteins if globular, then surface area is?
  12. 23. This enzyme is commonly used to cleave DNA into fragments for electrophoresis
Down
  1. 1. The gel commonly used in PAGE to separate proteins
  2. 2. Migration of charged particles in medium requires?
  3. 3. The Fragment of DNA with a known sequence used as a standard in electrophoresis
  4. 5. The buffer system often used to maintain protein structure during native PAGE
  5. 6. Reducing agent that breaks disulfide bonds in proteins
  6. 8. suppose there are 3 types of DNA- ssDNA ,dsDNA and ds plasmid and you are to carry out electrophoresis, which of these will have faster electrophoretic mobility?
  7. 10. Proteins can be separated using electrophoresis based on?
  8. 12. Gel component with lower acrylamide concentration for protein separation
  9. 13. poor medium causes
  10. 15. Used to form loading wells in a gel, removed after the gel has set
  11. 18. Type of material exhibiting electroendosmosis
  12. 19. Gradient formed in isoelectric focusing to separate protein
  13. 20. the parameter that determines the degree of ionization of organic compounds