Genetic engineering of human insulin

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Across
  1. 5. Originally, short 'sticky ends' were created at the ends of the cut bacterial plasmids by using _______ nucelotides
  2. 6. Enzyme from retroviruses that can produce single stranded DNA from RNA is called _________ transcriptase
  3. 9. Addition of DNA _________, DNA polymerase and primers can produce double stranded DNA from single stranded DNA
  4. 10. Cattle and ______ were slaughtered and insulin extracted from their pancreatic tissue
  5. 11. Hormone consisting of 51 amino acids, produced by beta cells of the pancreas
  6. 13. Insulin, being coded for by less than 200 bases means the gene is very _______
Down
  1. 1. Advantages of using GM produced human insulin include being more efficient, eliminating ethical concerns of using cattle/pig tissue and that it is ___________
  2. 2. Originally, short 'sticky ends' were created at the ends of the synthesised insulin gene by using _______ nucelotides
  3. 3. Human genome is very large, consisting of 300 ________ bases
  4. 4. Transgenic E. coli bacteria were grown at an industrial scale within a ___________
  5. 7. Fred _______ was a double Nobel prize winner for Chemistry
  6. 8. Before the human insulin produced by bacteria can be used, it has to be collected and then _________
  7. 12. DNA _________ enzyme was used to join the sticky ends of the synthesised insulin gene and the cut open plasmid