Genetics Lab 2 Puzzle

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Across
  1. 1. If you have a DNA concentration of 1000 ng/ul with a total volume of 100 ul and you add 200 ul of a sample with a DNA concentration of 1000 ng/ul, what is the new DNA concentration in ng/ul? (In words)
  2. 3. If you have a DNA concentration of 1000 ng/ul with a total volume of 15 ul, how much DNA do you have in total in ng? (In numbers, no comma)
  3. 5. How many pg are in 1 ng?
  4. 9. If you have a DNA concentration of 0.3456 ug/ul, how many ug do you have per ml?
  5. 10. If you have a DNA sample with a concentration of 5000 ng/ul and take 10 ul of it and mix it with 90 ul of TE buffer, what is the new concentration?
  6. 12. What we used as a blank.
  7. 13. The instrument used to measure absorbance.
  8. 15. If you have a DNA concentration of 1000 ng/ul and want to create a 50 ul dilution with a concentration of 120 ng/ul, how many ul of your original DNA sample should you add? (In words)
  9. 16. What we generate based on our standards to determine unknown DNA concentrations.
  10. 17. The concentration of DNA after performing a 1/2 dilution on a DNA sample of 50 ng/ul, in ng/ul? (In words)
  11. 18. If you have a DNA concentration of 6789 ng/ul, how many ug per ul do you have?
  12. 19. If you have a DNA concentration of 1000 ng/ul with a total volume of 50 ul and you add 50 ul of water to the sample, what is the new concentration in ng/ul? (In words)
  13. 20. Using known DNA concentrations, we can determine the concentration of _____ DNA samples.
Down
  1. 2. If you have a DNA concentration of 1000 ng/ul with a total volume of 50 ul and you add 50 ul of water, what is the total volume of liquid in the sample? (In words)
  2. 4. According to your line of best fit, an absorbance of 0.3 corresponds to a DNA concentration of 60 ng/ul and an absorbance of 0.15 corresponds to a DNA concentration of 30 ng/ul. What is your k-value? (In numbers)
  3. 6. How many ng are in 1 ug?
  4. 7. We measure DNA at what wavelength?
  5. 8. What you placed your sample into for the spec to read.
  6. 11. There is a direct relationship between absorbance and DNA concentration is ____'s Law
  7. 12. If you have a DNA concentration of 500 ng/ul and you added 3 ul of it to 47 ul of water, what is the new DNA concentration of the new sample? (In words)
  8. 13. The k value is the ____ of the line of best fit.
  9. 14. If you have a k-value of 0.003 and your absorbance of a 1/100 dilution of unknown DNA is 0.30, what is your original DNA concentration in ng/ul? (In words)
  10. 15. The method we used to create a series of standards of different concentrations.