Genetics Lab 4 Puzzle

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Across
  1. 2. The process of cloning involves inserting DNA into a ______ vector.
  2. 3. The bond between nucleotides.
  3. 5. If X-Gal is cleaved, which color will be present in your bacteria colony?
  4. 8. We shine this onto our agarose gel to visualize DNA.
  5. 9. The process of inserting a plasmid into bacteria is called ________.
  6. 11. If the gene insert is properly inserted into the vector, which color will the colony be after a blue/white screening?
  7. 12. The process of making copies of DNA in vivo is referred to as _____.
  8. 13. The process where bacteria are placed from ice to 42 degrees and then immediately back to ice to take up DNA.
  9. 16. The specific bacteria cells we use for transformation.
  10. 17. This molecule activates protein translation of our vector in bacteria.
  11. 19. The optimal temperature for DNA ligation (in words)
  12. 20. Vectors contain an antibiotic ________ gene to only allow cells that took up the vector to grow in media.
  13. 22. Linear DNA can become circular through ______.
  14. 23. If the gene insert is properly inserted into the vector, LacZ gene will be _______.
  15. 26. The LacZ gene produced this protein, which cleaves lactose-like sugars.
  16. 29. A gene of interest is first ______ via PCR before inserting it into a vector.
  17. 30. The name of the gene we amplified in PCR.
  18. 31. Our vector contains a(n) _______ nucleotide at the end to help the gene insert attach to the vector.
  19. 32. Disruption of this gene allows us to do blue/white screening of bacteria to see whether our gene of interest was inserted.
Down
  1. 1. To cut into the plasmid DNA for gene insertion, we use ________.
  2. 3. The name of the vector we used in class.
  3. 4. A feature of plasmids that initiates DNA replication within the bacterium.
  4. 6. To attach a DNA sequence to a vector, it must be ______.
  5. 7. This helps us visualize how far DNA has traveled on an agarose gel.
  6. 10. After inserting DNA into bacteria cells, they are placed in ____ media to recover.
  7. 11. If X-Gal is not cleaved, which color will be present in your bacteria colony?
  8. 14. Bacteria cells that can readily take up DNA are called ______ cells.
  9. 15. The formation of a phosphodiester bond between the 3' hydroxyl group and the 5' phosphate group of DNA is called this.
  10. 18. Bacterial DNA is ________.
  11. 21. The latin term for an experiment performed with a living organism.
  12. 24. The name for a lactose-like sugar we use to do a blue/white test.
  13. 25. Taq polymerase adds a(n) ________ nucleotide to the end of a PCR product.
  14. 27. DNA ligase uses _____ to form the bonds between nucleotides
  15. 28. Complementary sequences of DNA in the vector sticky ends and the inserted gene must first ______.