HISTOPATHOLOGIC TECHNIQUES

3. An automatic tissue processor that can do the first four steps in tissue processing, however it cannot perform decalcification.
6. Cutting off excess paraffin wax to form a four-sided prism.
8. of intercellular & extracellular water from tissues after fixation & prior to wax impregnation.
10. Removal of dehydrating agent from tissues to replaced it with a medium that will dissolve the wax with which the tissues is to be impregnated.
11. Mixture of highly purified paraffin and synthetic plastic polymers; melting point of 56-57 deg. celcius.
13. Usually contains glacia acetone acetic acid; for parts of cells.
15. Classified both as nuclear and histochemical fixative and it can preserve nuclear protein and mucopolysaccharide.

1. Removal of calcium ions or lime salts from the organic extracellular matrix, calcified collagen and surrounding tissues of bones.
2. A method recommended for urgent biopsies, for specimens with much air like lungs, brain, eyes, spleen and CNS.
4. Composed of 2 or more simple components is combined.
5. It can act both as dehydrating agent and clearing agent, and is extremely dangerous and toxic.
7. Involves removal of clearing agent from tissues so that it will replace by a medium that will fill all cavities and tissues spaces.
9. Requires 4 changes of wax at 15 minutes interval. May be completed in 1 hour.
12. Tissues should not be more than 4-5 mm thick except when processing lung specimens which require 2cm.
14. Highly flammable and carcinogenic. Can damage BM leading to Aplastic anemia.