Lecture 5 : NUCLEIC ACID SEQUENCING METHODS

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Across
  1. 4. _______ salt is used to modify ‘C’ bases along with the chemical used to modify the T+C bases in Maxum-Gilbert method.
  2. 5. _________ chemical is used to modify the T+C bases in Maxum-Gilbert method.
  3. 8. _________ chemical is used to modify the ‘G’ bases in Maxum-Gilbert method.
  4. 9. _________ of the template DNA is radiolabelled in Maxum-Gilbert method.
  5. 11. ________ chemical is used to separate the radiolabelled DNA strand in Maxum-Gilbert method.
  6. 12. ________ is also known as high-throughput sequencing methods. (Abbreviation)
  7. 14. _______NTPs are used in the Sanger’s method of nucleotide sequencing.
  8. 15. __________ sequencing method is otherwise called as chain termination method.
  9. 16. ________ chemical is used to modify the G+A bases in Maxum-Gilbert method.
Down
  1. 1. ___________ is the instrument used to do automated cycle sequencing.
  2. 2. _________ labelled in the Sanger’s method of nucleotide sequencing.
  3. 3. __________ chemical is used to break the modified bases in Maxum-Gilbert method.
  4. 6. _________ group is absent in the ddNTPs used in the Sanger’s method of nucleotide sequencing.
  5. 7. __________ sequencing method is otherwise called as Chemical method.
  6. 10. __________ labelled primers are used in the automated cycle sequencing method.
  7. 13. _________ nucleotide of the template DNA cannot be identified by the Maxum-Gilbert method.