Mammalian Cell Culture

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Across
  1. 1. Fuse with HeLa plasma membrane so that DNA is transported into the cell
  2. 4. A cell-impermeable toadstool mushroom toxin that binds actin
  3. 5. Green Fluorescent Protein detected by fluorescent microscopy
  4. 6. Distinguishing living cells from nonliving cells
  5. 8. Etched glass chamber used to count cells
  6. 9. Artificial introduction of material into cells, typically involving the uptake of DNA
  7. 10. Growth of cells in an artificial environment outside of the organism
  8. 12. Store stocks of cells to prevent the need to have cell lines in culture at all times
  9. 13. A DNA molecule used as a vehicle to transfer foreign genetic material into another cell
  10. 14. Liquid designed to support growth of cells outside of the organism
  11. 16. Amino Acid
  12. 19. Make free of MOST live bacteria
Down
  1. 2. The execution of cell culture procedures without introducing contaminating microorganisms from the environment
  2. 3. Refers to coverage of the flask by the cells
  3. 7. Make free of ALL live bacteria
  4. 9. Describes the behavior of cells in a fluid environment
  5. 11. Crosslinks amino acids to stabilize the cell for examination
  6. 15. A nonionic detergent that disrupts cellular membranes without disturbing protein-protein interactions established by formalde-fresh
  7. 17. Polar Amino Acid that coats the flask
  8. 18. Breaks bonds between cells and lysine