Protein Analysis & Manipulation

4. Most convenient host for expression
5. Charge on proteins after SDS detergent
6. Used to make samples sink into wells for electrophoresis
8. Absorb UV light at 280 nm (ex/ tyrosine & tryptophan)
10. Property of molecules that repel water molecules
12. Process of separating proteins from solution by reducing solubility
13. Technique used to detect specific proteins based on molecular weight and size
20. Liquid remaining after centrifugation
22. Who proposed the Central Dogma of Molecular Biology?
23. Technique used to separate proteins using a stationary phase packed in a column
26. Column chromatography based on size exclusion
28. Column chromatography based on specific binding
29. Solution used to remove bound molecules from a solid support in chromatography
30. Method used to quantify protein concentration based on absorbance of Coomassie Brilliant Blue dye

1. Polymer used as a matrix in gel electrophoresis
2. Blue dye used to visualize gel samples
3. Method for concentrating proteins by inducing precipitation from solution
7. Digests the cell wall
9. Provides appropriate pH for gel electrophoresis
11. Method of protein quantification including BCA and Bradford
14. Technique used to visualize proteins with a brown/silver result
15. Protein quantification method involving the analyzation of UV absorption
16. Column chromatography based on charge
17. What type of bond holds secondary structures in place?
18. Molecules that are 6x smaller than DNA
19. Gel layer in electrophoresis where proteins are separated by size
21. Method for protein concentration quantification based of the reduction of copper ions
24. Process in gel electrophoresis where proteins gather before entering the separating gel
25. Column chromatography based on hydrophobicity
27. Selectively capture target proteins