Proteins, Cancer and CRISPR

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Across
  1. 1. A type of blot lab used to check if a specific protein like Prdx1 is overexpressed in cells
  2. 4. A vital protein that, when mutated, cannot detect DNA damage and severely increases cancer risk
  3. 5. The drug development stage where the largest clinical trials are conducted to confirm safety and efficacy
  4. 8. Long chain of amino acids
  5. 10. Directed Repair (HDR); a high-fidelity DNA repair pathway used to replace a mutated sequence with a proper sequence
  6. 12. The drug development phase where therapies are tested on human cells and animals before human clinical trials
  7. 14. A substance used in PAGE electrophoresis to denature proteins and give them a negative charge
  8. 16. Mutated genes that, along with tumor suppressors, are directly linked to progressing cancer
  9. 18. The process of inserting a plasmid into bacteria, often induced by heat shock
Down
  1. 2. A traditional cancer treatment, along with radiation and chemotherapy
  2. 3. Disulfide bonds in a protein chain form specifically to connect two residues of this amino acid
  3. 4. The specific type of chemical bond formed between amino acids during dehydration synthesis
  4. 6. A chemical added to tightly control and induce protein expression in certain lab systems
  5. 7. Medical drugs or therapies that are derived directly from living organisms
  6. 9. A lab safety symbol indicating a chemical that can severely burn and damage skin or metal
  7. 11. The abbreviation for the Quality Control department in charge of testing product safety and potency
  8. 13. The gene-editing enzyme discovered in bacteria that cuts both DNA strands
  9. 15. A type of template DNA required only when using homology-directed repair to precisely fix a genome
  10. 17. The type of RNA (gRNA) that is complementary to the target sequence and directs where CRISPR will cut
  11. 19. The government agency responsible for keeping workers safe on the job