RECOMBINANT DNA TECHNOLOGY

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Across
  1. 4. What type of cell that is able to accept foreign DNA , that is the cloning vectors and allows the vectors to multiply?
  2. 6. Name a method to amplify DNA , that is, produce many copies of specific fragments of DNA in vitro.
  3. 7. Restriction enzymes cut DNA at specific sequences known as
  4. 10. Which enzymes function as s defence mechanism protecting the bacteria ?
  5. 12. A cloning vector which is small,circular DNA molecules which occur naturally in addition to the bacterial chromosome
  6. 14. What step is this called when the DNA fragment is then cooled to allow Taq polymerase and primers to bind to the DNA ?
  7. 17. The plasmid that carries a foreign gene is known as a
  8. 18. Name one of the prokaryotic cell.
Down
  1. 1. The process of bacteria protect their own DNA from their restriction enzymes by adding methyl groups to the adenine or cytosine within the restriction site is called
  2. 2. Are bacteria-infecting viruses.
  3. 3. State one types of restriction enzymes.
  4. 5. How many stages involved in gene cloning ?
  5. 8. When DNA recombination occurs randomly ?
  6. 9. Give one example of cloning vectors.
  7. 11. How many types of plasmids produced when the DNA fragments are then joined by the enzyme DNA ligase which forms covalent bonds between nucleotides ?
  8. 13. is a combination of uekaryotic chromosomes (yeast) and foreign dnas.
  9. 15. How many tools used in Recombinant DNA Technology ?
  10. 16. Which ions are used to induce the permeability of the bacterial cell walls ?