RECOMBINANT DNA TECHNOLOGY

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Across
  1. 4. a large collection of cloned DNA sequences from a single genome
  2. 7. vectors are chosen depending on the _______ of DNA that must be included in a library
  3. 8. the DNA fragments are cut out of their normal position in the chromosome using _______ enzymes
  4. 10. most common application of recombinant DNA
  5. 11. contains DNA present in a given cell population which is prepared from the mRNA(messenger RNA) using the enzyme reverse transcriptase
  6. 12. the small replicating molecule in cloning is called a DNA _________
  7. 14. recombinant DNA is possible because DNA molecules from all organisms share the same ________ structure
  8. 15. vector based on F-factor (fertility factor) plasmids of E. coli
Down
  1. 1. not a part of the main cellular genome,but they can carry genes that provide the host cell with useful properties and commonly found
  2. 2. a recombinant variety of rice that has been engineered to express the enzymes responsible for ß-carotene biosynthesis
  3. 3. proteins are widely used as _________ in laboratory experiments and to generate antibody probes for examining protein synthesis within cells and organisms
  4. 5. DNA molecules formed by laboratory methods of genetic recombination
  5. 6. a group of individual cells or organisms descended from one progenitor
  6. 9. dna is _________ from the organism under study and is cut into small fragments of a size for cloning
  7. 13. vector based on autonomously replicating plasmids of Saccharomyces cerevisiae
  8. 14. engineered vectors that are hybrids of plasmid and phage lambda