Recombinant DNA Technology
Across
- 1. use a DNA sequence to find complementary DNA or RNA sequences in a gel via hybridization.
- 3. a type of blot that uses a give antibody to identify proteins of interest.
- 7. identify the specific nucleotide sequence of a piece of DNA
- 9. AKA restriction enzyme, naturally occurring enzyme that cuts up DNA sequences at specific regions called restriction sites.
- 13. DNA molecules that accept foreign DNA to be replicated
- 16. the cell that replicates the vector and foreign DNA
- 17. creates a map of restriction sites.
- 18. making identical copies of a specific DNA molecule
Down
- 2. Basic local alignment search tool
- 4. 1. source of DNA 2. Endonuclease/restriction enzyme 3. Vector 4. Host Cell
- 5. a type of library that preserves the entire genome of an organism.
- 6. collections of recombinant phage or bacteria that each contain a different cloned piece of DNA from the same organism.
- 8. DNA created by joining together pieces of DNA from different sources
- 10. a type of blot that uses a DNA sequence to identify complementary RNA sequences of interest.
- 11. primers that are single stranded DNA about 10-20 bases long; act as a starting piece for the replication of the strand in PCR.
- 12. a type of blot that uses a DNA sequence to identify complementary DNA sequences of interest.
- 14. any DNA or RNA sequence that is complementary to some part of a cloned sequence present in the library' has a label or tag so it can be identified when it finds the match.
- 15. a type of library where the DNA sequences that are preserved in the bacteria/phage, are complementary to the mRNA that was being produced in a cell when the sample was taken.