Recombinant DNA technology

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Across
  1. 2. REases are classified into these many main types of enzymes
  2. 6. Group of identical copies of some biological entity
  3. 9. Type of enzyme which generates DNA from RNA
  4. 10. Host DNA marking which can prevent it from undergoing degradation by REases
  5. 12. Molecule that will be used to introduce the sequence of interest into a living host cell
  6. 13. Primers used to capture mRNA from eukaryote cells due to their characteristic PolyA tail
  7. 14. Seeks to achieve the retrieval of bacteria which contain specifically recombined vectors
Down
  1. 1. Restriction enzymes attack this type of bond
  2. 3. Many REases require this divalent metal cofactor for their activity
  3. 4. Enzyme used to join DNA ends together
  4. 5. Fundamental technique in recombinant technology; the making of multiple copies of a DNA sequence
  5. 7. Ends in which one strand is longer than the other (typically by at least a few nucleotides), such that the longer strand has bases which are left unpaired
  6. 8. An enzyme that cleaves DNA into fragments at or near specific recognition sites
  7. 11. Type of hybrid plasmid that contains a Lambda phage cos sequence