Summer Training - 2017

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Across
  1. 2. In SDS-PAGE, protein molecules are separated based on its
  2. 4. The DNA intercalating dye used in real-time PCR is
  3. 8. At 260 nm wave length, ___________ molecules absorbs the maximum
  4. 10. Laminar Air flow hoods used in microbiology laboratory belong to _______ type biosafety cabinet
  5. 11. In Gram’s stain, Gram positive bacteria stains
  6. 13. Direct ELISA is used detect
  7. 14. Polymorphic DNAs can be differentiated by using the molecular technique _______; Abbreviation
  8. 16. In cell culture, _________ is added to maintain the pH of the cell culture medium
  9. 17. For isolation of viruses from chicken, ________ is most commonly used; Abbreviation
  10. 18. Most commonly used enzyme in ELISA is;Abbreviation
  11. 19. At 280 nm wave length, ___________ molecules absorbs the maximum
  12. 22. Enzyme used in PCR for DNA amplification is
  13. 27. Widal test is an example of _________ test
  14. 30. In Gram’s stain, Gram negative bacteria stains
  15. 31. The salt used to prepare the McFarland solution is
  16. 33. ___________ is used to add as a cryoprotectant in the cryopreservation medium of cells; Abbreviation
Down
  1. 1. In cloning, _________ is the enzyme used to combine the ends of the vector and foreign DNA
  2. 3. For identification of microorganisms by PCR and gene sequencing, _________ is used as a target gene
  3. 5. Enzyme used to convert RNA into cDNA is
  4. 6. In lateral flow strip test, presence of control line and test line indicates that the sample is
  5. 7. Transferring protein molecules from SDS-PAGE to nitrocellulose membrane is called
  6. 9. Selective growth of Gram negative bacteria can be achieved by adding ________ to media
  7. 12. In genomic DNA extraction from bacteria, __________ substance is used to break the cell wall
  8. 15. _________ is an oligonucleotide usually tagged with a reporter molecule and used in nucleic acid hybridization technique for identification of organisms
  9. 20. ___________ is used to disaggregate the cells for subculturing the cells
  10. 21. Stain used to identify the amplified DNA of PCR in agarose gel is
  11. 23. In vitro amplification of target nucleic acid is called; Abbreviation
  12. 24. _______ is the probe used in real-time PCR
  13. 25. _________ is used to identify either antigen or antibody based on the presence of line between the wells; Abbreviation
  14. 26. ___________ is lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism following overnight incubation; Abbreviation
  15. 28. In agarose gel electrophoresis, DNA molecules move towards _________ end (anode) when apply current
  16. 29. Haemagglutintion inhibition (HI) test is done to measure the amount of _______ present in the sample
  17. 30. The extrachromosomal DNA of bacteria is called known as
  18. 32. The ________ is defined as the number of cycles required for the fluorescent signal to cross the threshold