Techniques in Molecular Biology

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Across
  1. 4. Short oligonucleotides use for hybridization technique.
  2. 7. Solvent used to precipitate DNA from crude extract.
  3. 8. Molecule which have high affinity for Avidin.
  4. 12. Polymerase enzyme used for PCR reaction.
  5. 13. The amplified products of PCR reaction.
  6. 14. Chemical use for cleavage of DNA after modification for sequecing of DNA by chemical cleavage method.
  7. 15. DNA sequencing method based on sequencing by synthesis.
  8. 16. Enzyme used to digest the cell wall of bacteria for isolation of plasmid DNA.
  9. 17. an In-situ hybridization technique.
  10. 19. The process of binding of primer to the denatured strand.
Down
  1. 1. Non-ionic detergent that can precipitate Nucleic acids.
  2. 2. Protein denaturant.
  3. 3. Gel use for separation of DNA using electrophoresis.
  4. 5. Blotting technique used to identify a DNA.
  5. 6. The purines and pyrimidines bases in DNA absorb light in the _____ region of the light spectrum.
  6. 7. Technique use for detection of Radioactively labelled DNA.
  7. 9. An ATPase that catalyzes the conversion of luciferin to oxyluciferin
  8. 10. Technique which measures Genetic relatedness between two species using their DNA.
  9. 11. Step in PCR in which new nucleotides are added to the primer
  10. 18. Fluorophore used for labeling of primer.