Topic 14. Stem Cells

123456789101112131415
Across
  1. 1. Pluripotent stem cells can be identified using distinct surface ______ to indicate the extent of differentiation.
  2. 3. In _________ cloning, nucleus from patient’s somatic cell is transfer to a enucleated oocyte from a donor to generate an embryo that can provide stem cells that is immunologically compatible with donor patient for transplantation
  3. 5. Hematopoietic stem cells are multipotent blood stem cells comprising of the _____ and myeloid progenitor cells
  4. 6. The formation and thickening of the fertilization envelop that surrounds the fertilised egg is caused by the_______ of hydrolytic enzymes stored in vesicles.
  5. 8. Stem cell lines developed in the lab need to undergo pluripotency assessment by ________test, comparing transcriptomic profile of the derived cell line with a reference stem cell in the database to rule out contamination as well as genetic and epigenetic abnormality that affects the gene expression pattern.
  6. 10. Umbilical cord can be a source of multipotent stem cells which can be frozen for future therapeutic use and for ___________transfer.
  7. 11. _________stem cells are derived from 1-2 cell stage of an embryo and have unlimited ability to form other cell or tissue types and even the embryo itself.
  8. 12. The first cell cycles of fertilized egg are driven by ________ mRNA and proteins in the egg whereas subsequent cell divisions and protein synthesis are driven by the embryonic gene activation
  9. 13. Besides fibroblasts, ________can be reprogrammed into adult stem cells.
  10. 14. Interspecies mating is prevented due to the _______ binding of protein on sperm to the receptor on egg cell.
Down
  1. 2. When B-cell receptor (BCR) is autoreactive, SLP65 inhibit signal transduction, FOXO is unphosphorylated which will upregulate the transcription of Rag 1 and Rag 2 for activation of Ig light chain gene recombination leading to B-cell receptor class _________ and B cell differentiation or maturation.
  2. 4. An alternate approach for tissue therapy is to____________ terminally differentiated cells to dedifferentiate into multipotent proliferative cells with the capacity to regenerate all components of a damaged tissue.
  3. 7. For safety purposes, it is important to test the induced multipotent or pluripotent stem cells for ___________ potential or neoplastic growth.
  4. 9. Stem cells renewal mechanisms include obligatory ___________replication and stochastic differentiation
  5. 15. The __________ of stem cells decreases when cells differentiate.