Untitled

12345678910111213141516171819202122232425262728293031323334353637383940414243444546474849505152535455565758
Across
  1. 2. A chromate fixative that preserves lipids and mitochondria.
  2. 4. Round to oval shaped cells with translucent basophilic cytoplasm due to glycogen accumulation.
  3. 5. This is for early degenerative processes and tissue necrosis.
  4. 8. ACID It is a moderate-acting decalcifying agent which produces better nuclear staining.
  5. 12. Examples of this method are the Heat Fixation and Microwave Technique.
  6. 13. It contains mercuric chloride and glacial acetic acid.
  7. 14. Also known as Moller’s Fluid.
  8. 15. It refers to the failure of organ to reach normal mature adult size.
  9. 17. This process is recommended for making small sections of celloidin blocks.
  10. 21. This is recommended for clearing embryos, insects and very delicate specimens.
  11. 24. It consisted of 3 stains, developed by George Papanicolau, the father of cytopathology.
  12. 26. This type of alcohol is recommended for touch preparations.
  13. 28. It is synthetic wax substitute similar to Paraplast with a melting point of 56-58°C.
  14. 31. It does not always increase the size of an organ.
  15. 32. A water soluble wax for enzyme histochemistry.
  16. 36. It is a bridging discipline devoted to the study of the structure and functional changes in cells, tissues and organs that underlie a disease.
  17. 37. A purified form of nitrocellulose that is soluble in alcohol and ether.
  18. 38. This rapidly denatures and precipitates proteins.
  19. 40. The product of paraffin with rubber.
  20. 44. Developed at national Cancer Institute in December 1988.
  21. 46. A way of cutting off excess paraffin wax to form a 4 sided prism.
  22. 47. EDTA One of its advantages include permitting excellent staining results.
  23. 52. This is an efficient substitute for xylene, as it is non-hazardous, less expensive and causes less shrinkage of the tissue.
  24. 53. It is considered as reversible process.
  25. 55. Used for fixing wet and dry smears, blood smears and BM tissues.
  26. 56. A method in which cells are collected using various tool from body surfaces.
  27. 57. This is utilized in plant and animal micro-techniques.
  28. 58. Boat shaped cells with a tendency to fold or curl on edges.
Down
  1. 1. In this, blocks produced are even, with parallel sides, and with a fairly shaped initial setting of the wax.
  2. 3. Classified both as nuclear and histochemical fixative.
  3. 6. Secondary changes in somatic death that occurs 6-12 hours after death.
  4. 7. It is often sold as a xylene replacement.
  5. 9. Recommended only for frozen tissue section, histochemical and enzyme studies.
  6. 10. It is a mixture of highly purified paraffin and synthetic plastic polymers, with a melting point of 56-57°C.
  7. 11. This fixative is recommended for preservation of enzymes like lipases and phosphatases.
  8. 12. It is the fried egg appearing cells.
  9. 16. It refers to incomplete or defective development of tissue or organ.
  10. 18. It is the vascular response to injury.
  11. 19. It is the study of tissues
  12. 20. This fixative is recommended for fixing chromosomes and lymph glands.
  13. 22. The most common metallic fixative.
  14. 23. It is the first secondary change to appear; important in establishing time of death.
  15. 25. This reagent causes minimum shrinkage of tissues.
  16. 27. In this dehydrating agent, the tissues tend to ribbon poorly.
  17. 29. It is a pathological process which is detrimental to the body.
  18. 30. It involves wax impregnation under negative atmospheric pressure inside an embedding oven.
  19. 31. It is an increased of production of cellular proteins.
  20. 33. It is a normal, natural process which is necessary for the body as it maintain the balance in the number of cell in the body.
  21. 34. It is used in routine tissue processing.
  22. 35. It is inferior compared to nitric acid in its role as a decalcifying agent.
  23. 39. It is a failure of an organ to form an opening.
  24. 41. The first and most critical step in histotechnology
  25. 42. A wax with a much lower melting point 46-48oC
  26. 43. It is recommended for enzyme histochemistry and EM.
  27. 45. Purplish discoloration of skin due to sinking of fluid blood into capillaries in the parts of the body.
  28. 48. This dehydrating agent can dehydrate and clear tissues at the same time.
  29. 49. This is better at preserving tissue structure than xylene.
  30. 50. It is a colorless clearing agent that is most commonly used in histology laboratories.
  31. 51. It refers to complete non-appearance or absence of an organ.
  32. 54. It affect or alter the cellular and tissue morphology/loss of structures.