Across
- 3. for GIT biopsies & endocrine tissues
- 5. it is consist of 3 stains, developed by George Papanicolau, the father of cytopathology
- 8. Type of microtome for preparaing paraffin section
- 10. it is an example of large specimen that must be sectioned or with fixative
- 12. These tools are needed for handling sections during cutting
- 13. The thermostatically controlled type is preferable, but if is unavailable, water from a hot water tap can be used although this can give rise to air bubbles which may be trapped under cut sections.
- 15. due to extravascular accumulation of fluid.
- 16. These are thin slices of tissues are produced during sectioning
- 17. study of tumors or neoplasm
- 19. involves getting tissue sample from a living person
- 20. Excellent for: Glycogen demonstration
- 22. It is a method wherein it removes the formalin pigments; contains Hydrogen peroxide, 28% ammonia water, and Acetone
- 23. It is an alcohol fixation that focuses on connective tissue / CT mucins & umbilical cord
- 24. it is liquid at room temperature
- 26. Side that is concave is for cutting paraffin embedded tissues and the flat is for cutting celloidin embedded tissues.
- 27. allows the general microscopic study of tissue structures without altering the structural pattern and normal intercellular relationship of tissues
- 30. absence of ear canal
- 31. should expand the section to its original dimensions and ensure that it is completely flat.
- 33. involves the removal of gross nicks on the knife edge (Coarse Honing) to remove blemishes, and grinding the cutting edge of the knife on a stone to acquire an even edge
- 35. He invented Rotary Micrtome in 1885 to 1886 to cut paraffin embedded tissues
- 39. preserve chemical components of tissues like enzymes
- 41. common term for malignant tumors
- 43. recommended for enzyme histochemistry and EM (Electron Microscopy)
- 44. gives more polishing effect than Belgium yellow
- 45. for early degenerative processes and tissue necrosis; used for rickettsia and other bacteria
- 47. He is the one who developed sliding microtome in 1789
- 48. it is formed during honing is removed and the cutting edge of the knife is polished.
- 50. Father of Modern Pathology
- 51. recommended for fixation of embryos, pituitary biopsies and endometrial curettings. NOT for kidneys & Abolishes feulgen’s reaction; not compatible
- 52. drying & wrinkling of the anterior chamber of the eye and cornea
- 55. This medium is used for methylene blue-stained nerve preparations and as a general purpose aqueous mountant.
- 57. Increases movement of molecules thereby accelerating fixation, staining, decalcification, immunohistochemistry and EM
Down
- 1. process of polishing and sharpening the cutting edge.
- 2. seen in acute pancreatitis; affected organs appears like chalky white precipitates
- 4. Type of microtome which is for frozen section
- 6. It should be adjusted down to the lowest level possible because too high a concentration may adversely affect the tissue and produce artefact similar to that caused by excessive heat.
- 7. It has a high index of refraction and provides greater visibility if slightly diluted with water (for moist sections)
- 9. type of knives that are used to cut any type of resin block for electron microscopy.
- 11. Aerosol sprays
- 14. An automatic tissue processor that can do fixation, dehydration, clearing, and infiltration
- 18. It should never come in contact with a strop because it blisters and destroy the leather.
- 21. A fixation method in which it uses chemical fixative such as 10% formalin
- 22. It is where hard tissue must undergo; immersing specimen in 4% aqueous phenol for 1-3 days
- 25. must not be allowed to come in contact with the strop
- 28. This fluid is recommended for mounting frozen sections from water.
- 29. looks for abnormalities in tissues
- 32. Result color of Mycelia in Pap Stain
- 34. it is a fixative that usually contain glacial acetic acid as their primary component due to its affinity for nuclear chromatin.
- 36. these are tissues take a longer to infiltrate it must be left to infiltrate.
- 37. type of microtome that is equipped with a glass or gem grade diamond knife is used to cut very thin sections of tissue embedded in epoxy resin.
- 38. supplied as a 40% aqueous solution; smallest aldehyde fixative
- 40. these knives are generally used for trimming and semi-thin sectioning of tissue blocks for electron microscopy
- 42. atypical cells present, but negative for malignant cells
- 46. Recommended for preservation of enzymes (lipases and phosphatases) & for fix brain tissues for the diagnosis of rabies
- 49. Recommend for preserving carbohydrates
- 51. 120mm long, both sides are concave, used to cut paraffin embedded tissues.
- 52. pain due to the release of chemical substances like bradykinin that stimulates nerve endings for pains.
- 53. Recommended for mailing specimens since it is a tolerant fixative
- 54. procedure wherein the excess wax is cut off from the block to expose the tissue surface in preparation for actual cutting
- 56. A Section which may be cut from tissues that have been fixed and frozen with CO2 or for fresh or fixed tissues frozen with the cryostat.