Modification of Spontaneous Colicin Production

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Across
  1. 3. The DNA region that controls the transcription of colicin genes.
  2. 6. The transcriptional repressor that prevents colicin gene expression under normal conditions.
  3. 7. The type of attack strategy where colicin production is triggered by a competitor's presence.
  4. 8. The method used to measure colicin expression levels in individual cells.
  5. 9. The overall process of controlling colicin expression through genetic mechanisms.
  6. 12. A protein that binds to colicin and prevents it from killing the producing bacteria.
  7. 14. The receptor required for colicin uptake into bacterial cells.
  8. 17. The technique used to quantify fluorescent cells in this experiment.
  9. 19. The toxin produced by E. coli to attack competitors.
  10. 20. The immunity protein for colicin E2, which neutralizes its toxic effects.
Down
  1. 1. The effect of high immunity protein levels on autoinduction and colicin production.
  2. 2. The process by which the immunity protein binds colicin to prevent self-toxicity.
  3. 4. The type of plasmid used to measure colicin promoter activity via GFP expression.
  4. 5. The level of colicin expression seen in the control (E2R) strain.
  5. 10. The strain containing only the colicin E2 reporter plasmid, serving as a baseline control.
  6. 11. The process where colicin presence triggers more colicin production.
  7. 13. A genetic change that can alter colicin or immunity protein regulation.
  8. 15. The type of attack strategy where colicin is produced even before a competitor is detected.
  9. 16. The strain that contains both the colicin E2 reporter and colicin plasmid, showing increased fluorescence.
  10. 18. The bacterial stress response that regulates colicin production.