VPH 83 Crossword Puzzle

6. Objective state of meat decay observed when carcass pH reaches or exceeds 6.8.
8. term for an animal that died prior to exsanguination.
9. Solidifying substance in pour plates and selective diagnostic media.
12. Exterior part of the meat cube harboring higher microbial contamination.
14. Differential agar medium used to isolate Gram-negative enteric bacteria.
15. Thermal technique used to pass glass slides through a flame to fix bacteria.
17. Systematic reduction of bacterial density using water blanks (e.g., 1:100, 1:1000).
20. "Killed in ____"; condition resulting in cloudy, olive-green test results.
21. Visible mass of bacteria arising from a single cell on an agar plate.
22. Energy store in living muscle converted to lactic acid post-mortem.
23. Bleeding efficiency test devised by M. Radan using malachite green.
25. component providing oxygen for the pseudo-peroxidase reaction.
26. Solvent used in Exercise 2 to dissolve fat from glass slides prior to staining.
28. Objective (100x) required to examine bacterial smears.

1. Concentrated chemical added to acidify the malachite green/peroxide mixture.
2. Action of maintaining agar plates at 37°C for 2 to 5 days.
3. Green reagent forming an oxyhemoglobin complex in the presence of blood.
4. Sterile cotton instrument used to spread 0.1 mL of diluted sample.
5. Non-bacterial contaminants enumerated alongside bacteria on pour plates.
7. Muscle protein coagulated by lactic acid during the onset of rigor mortis.
10. classification for high-pH meat restricted to canning.
11. Glassware dish used for mincing tissue or supporting pour plates.
13. Internal muscle tissue sampled with a second sterile knife to test deep sterility.
16. Enzymatic breakdown of muscle causing post-mortem pH to rise slowly.
18. Organic acid that lowers carcass pH from ~7.2 down to 5.4–6.0.
19. Four-letter acronym for thiosulfate-citrate-bile salts-sucrose agar.
24. Removal of blood from the carcass; essential for extending meat shelf life.
27. dye used for 1 minute during direct microscopic examination.
29. post-mortem hardening and contraction of muscular tissue.