Anaerobic Cultures: General

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Across
  1. 3. in an anaerobic indole test, this reagent must be used in place of Kovac's reagent
  2. 7. thioglycollate, cysteine, and dithiothreitol are all __________ agents added to media to culture anaerobes
  3. 9. anaerobic organisms lack both catalase and this enzyme
  4. 12. this type of organism requires oxygen, but at much lower concentrations than found in typical aerobic environments
  5. 14. this kind of test can distinguish Clostridium (-) from Bacillus (+) and Propionibacterium (+) from Lactobacillus (-)
  6. 15. __________ anaerobic specimens include: abscesses of the brain, liver, lung, and abdomen, insect bites, necrotic tissues, sterile-site fluids, and wounds near mucous membranes
  7. 16. this type of anaerobe can grow with some oxygen exposure, but does best in anaerobic conditions
  8. 18. the presence of this enzyme causes an opaque white, diffuse zone on egg yolk agar caused by the breakdown of lecithin into diglyceride and phosphotylcholine
  9. 19. this type of anaerobe identification is based on the morphology of colonies, gram stains, and biochemical testing
Down
  1. 1. this type of anaerobe can grow in both aerobic and anaerobic environments
  2. 2. the gold standard for the definitive identification of anaerobes is gas-liquid __________
  3. 4. an anaerobe that grows best in a carbon dioxide environment
  4. 5. this trait of some anaerobic organisms can be observed under UV light
  5. 6. the antimicrobial disks used to help identify anaerobes are vancomycin, kanamycin, and __________
  6. 8. the presence of this enzyme causes an iridescent sheen on colonies that is due to the hydrolysis of triglycerides into glycerol and fatty acids
  7. 10. the method of collecting anaerobic specimens should focus on reducing the amount of contamination from __________
  8. 11. this kind of disk is used to distinguish anaerobic gram negative rods, especially B. fragilis and Prevotella
  9. 13. __________ anaerobic specimens include: nasopharyngeal or throat swabs, vaginal swabs, urine and stool, and surface wound swabs
  10. 17. this kind of anaerobe must have an environment with less than 0.5% oxygen