Animal Tissue Culture

3. A culture technique that involves immortalized cells from a single clone
5. The state when cells completely cover the surface area of the culture vessel.
8. A type of cell line that can be passaged indefinitely and does not die after a certain number of divisions
9. Equipment used to ensure sterility during cell handling, often called a biosafety cabinet.
12. The process of transferring cells from one culture vessel to another
14. A type of microscope used to observe adherent cells growing at the bottom of culture flasks or dishes.
17. The process of freezing cells at very low temperatures for long-term storage.
18. The medium that supports cell growth and often includes serum
19. Transparent vessel commonly used for observing cell growth under a microscope.

1. An enzyme commonly used to detach adherent cells during subculturing.
2. Used in laboratories to measure the effects of compounds or substances on cultured cells
4. A single, confluent sheet of adherent cells covering the surface of a flask or dish.
6. A commonly used reagent in media that provides growth factors, often obtained from bovine sources.
7. Culture that is derived after the first subculturing from the primary culture.
10. This type of cell culture grows from established immortalized cellsINCUBATOR An essential piece of equipment used for incubating cell cultures at specific temperatures
11. A crucial ingredient in the media that provides energy to cells
12. A type of mammalian cell culture where cells are grown directly from tissue
13. A cell culture medium supplement that helps maintain pH
15. CELLS The first immortal human cell line, derived from cervical cancer.
16. Reagents often added to media to reduce the risk of bacterial contamination.