Across
- 4. The removal of the nucleus of one cell and insertion into an egg cell where the nucleus has been removed.
- 8. DNA sequence recognized by a restriction enzyme.
- 11. The use of a labeled (fluorescent or radioactive) sequence of RNA or DNA that is complementary to the sequence one wants to detect and localize. The result? Pretty and colorful data.
- 12. Purposely altering a DNA sequence outside of an organism and then putting it back into an organism to see what happens. Yep, mutations can even be intentional.
- 13. How the heritable information in a gene is manifested in the cell. Much like a circuit breaker, this can describe whether a gene is turned on or off
- 15. Pluripotent Cells Already differentiated cells that can be coaxed in the lab to rediscover themselves and become cells that have the potential to differentiate into any type of cell
- 17. An enzyme that acts like a glue connecting the phosphate backbone of a piece of DNA inserted into a cloning vector. Not the type made by Elmer's.
- 18. Cells Cells that have already been programmed to be a certain type of cell. They know what they want to be when they grow up, and there's no stopping them.
- 19. The insertion of a gene of interest into a souped-up vehicle or vector that allows a single gene to be studied.
- 20. A plasmid is a small piece of DNA, maintained separately from the chromosome. Plasmids are common in prokaryotes, but rare in eukaryotes.
Down
- 1. The use of an electrical current applied to a gel matrix to separate biological molecules.
- 2. Any food that is, whole or in part, produced with crops that have had modifications made to their genetic material.
- 3. The use of an electrical current to create small holes in a cell membrane. Used to introduce recombinant DNA into a cell.
- 5. Modified Organisms Organisms in which foreign genes have been introduced for some benefit. These benefits may include cows producing milk with more protein, plants resistant to herbicides, and peas made to not be so, um, mushy. Well, maybe not the last one…yet.
- 6. The technique used to amplify smallamountofDNA Restriction Site
- 7. The determination of the order of nitrogenous bases in DNA.
- 9. A circular piece of DNA that has been souped-up for the easy insertion and expression of a piece of DNA.
- 10. Changing the DNA to add one or more traits.
- 14. Cells that have the potential to become any type of cell. Like many students, these cells haven't quite figured out what they want to be when they grow up.
- 16. chip to identify which sequences are present in a sample.
