Biology II Microbiology Fundamentals

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Across
  1. 1. Keeping cultures at a controlled temperature and environment to allow microorganisms to grow.
  2. 3. (two words) The physical appearance of a colony (size, shape, color, texture, margin, and elevation).
  3. 10. (two words) A test condition where no response is expected (e.g., sterile swab plated without sampling).
  4. 12. (two words) The plan of an experiment that identifies variables, controls, and procedures.
  5. 15. A gel made from seaweed, used as a solid growth surface for culturing microbes.
  6. 19. (two words) A process of problem-solving that includes observation, hypothesis, experimentation, analysis, and conclusion.
  7. 22. A sterile cotton tip on a stick used to collect samples of microorganisms from surfaces.
  8. 23. The complete removal or destruction of all living microorganisms (e.g., by flame, autoclave, or chemicals).
  9. 24. Heating a metal tool (like an inoculating loop) until red-hot to sterilize it.
  10. 25. (two words) The factor that is measured in an experiment as it responds to changes in the independent variable.
Down
  1. 2. (two words) A wire tool with a small loop at the end, used to transfer microorganisms to agar or broth.
  2. 4. (three words) A single bacterium or small group of bacteria that gives rise to one visible colony.
  3. 5. (two words) A method of spreading bacteria across an agar surface in quadrants to isolate individual colonies.
  4. 6. (two words) A test condition where a known response is expected, ensuring the experiment works as intended.
  5. 7. (two words) Laboratory practices used to prevent contamination of samples and protect people from exposure to microbes.
  6. 8. A testable prediction about how variables in an experiment are related.
  7. 9. A visible cluster of microorganisms growing on the surface of solid media, usually arising from a single cell.
  8. 11. Unwanted growth of organisms in a culture or experiment due to poor sterile technique.
  9. 13. Repeating an experiment or trial to confirm results are reliable and not due to chance.
  10. 14. Factors in an experiment that can be changed, controlled, or measured.
  11. 16. (two words) A shallow dish with a lid, used for culturing microorganisms on agar.
  12. 17. A small portion collected from the environment to test or observe in an experiment.
  13. 18. (two words) A jelly-like medium containing nutrients to support bacterial growth.
  14. 20. (two words) A baseline test condition where the experimental variable is not applied, used for comparison.
  15. 21. (two words) The factor that is intentionally changed by the experimenter.