Biosafety

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Across
  1. 1. Cardboard boxes should not be stored or used in cold rooms or fridges due to the risk of this condition.
  2. 3. Glassware contaminated with this type of material requires disposal as sharps waste.
  3. 7. Many commonly used research viral vectors are intentionally designed to lack the ability for this process, reducing the likelihood of producing infectious virus.
  4. 9. Filter syringes, even without needles, should be discarded in this puncture-resistant waste container.
  5. 10. Face protection that should be used in addition to safety glasses when working with Liquid Nitrogen.
  6. 12. Gloves must be removed before touching this common lab exit point.
  7. 14. The most frequently changed piece of PPE in a BSL-2 lab.
  8. 15. Committee responsible for reviewing research involving animals.
  9. 16. Open manipulation of human blood should occur inside this primary containment device.
  10. 17. RMW containers should be sealed when they reach approximately this fraction of their capacity to prevent overfilling.
  11. 20. Before being placed in RMW, tissue culture plasticware should be treated using freshly prepared 10% bleach.
Down
  1. 2. Before handling materials in a BSL-2 lab, this garment should be donned to protect clothing and skin.
  2. 4. To verify that containment and airflow functions remain within required specifications, Biosafety Cabinets should be recertified at what routine frequency?
  3. 5. Process used to evaluate hazards and implement mitigation measures before work begins.
  4. 6. After removing gloves, perform this important hygiene practice.
  5. 8. Dry ice or liquid nitrogen sealed within ampules or vials may generate this hazard as temperatures increase and pressure builds.
  6. 11. Committee responsible for reviewing recombinant DNA and other biohazardous research.
  7. 13. Acronym for the primary containment device used when conducting aerosol-generating procedures.
  8. 18. Contaminated culture plates are treated prior to disposal as this type of waste.
  9. 19. A centrifuge safety feature used to contain aerosols.