Biosafety Crossword Puzzle

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Across
  1. 1. Contaminated culture plates are treated prior to disposal as this type of waste.
  2. 2. RMW containers should be sealed when they reach approximately this fraction of their capacity to prevent overfilling.
  3. 5. Before being placed in RMW, tissue culture plasticware should be __________ using freshly prepared 10% bleach.
  4. 7. Dry ice or liquid nitrogen sealed within ampules or vials may generate this hazard as temperatures increase and pressure builds.
  5. 9. After removing gloves, perform this important hygiene practice.
  6. 11. Face protection that should be used in addition to safety glasses when working with Liquid Nitrogen.
  7. 12. Committee responsible for reviewing recombinant DNA and other biohazardous research.
  8. 16. Process used to evaluate hazards and implement mitigation measures before work begins.
  9. 19. Before handling materials in a BSL-2 lab, this garment should be donned to protect clothing and skin.
Down
  1. 1. Many commonly used research viral vectors are intentionally designed to lack the ability for this process, reducing the likelihood of producing infectious virus.
  2. 3. Committee responsible for reviewing research involving animals.
  3. 4. Glassware contaminated with this type of material requires disposal as sharps waste.
  4. 6. Open manipulation of human blood should occur inside this primary containment device.
  5. 8. A centrifuge safety feature used to contain aerosols.
  6. 10. The most frequently changed piece of PPE in a BSL-2 lab.
  7. 13. Filter syringes, even without needles, should be discarded in this puncture-resistant waste container.
  8. 14. To verify that containment and airflow functions remain within required specifications, Biosafety Cabinets should be recertified at what routine frequency?
  9. 15. Acronym for the primary containment device used when conducting aerosol-generating procedures.
  10. 17. Cardboard boxes should not be stored or used in cold rooms or fridges due to the risk of what?
  11. 18. Gloves must be removed before touching this common lab exit point.