Biotechnology Colt Madron

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Across
  1. 1. Organisms in which a desired gene sequence has been inserted and expressed
  2. 3. field that generates useful products by manipulating living systems and their components
  3. 4. The use of an electrical current applied to a gel matrix to separate biological molecules
  4. 5. The use of an electrical current to create small holes in a cell membrane Used to introduce recombinant DNA into a cell
  5. 9. A circular piece of DNA that has been souped-up for the easy insertion and expression of a piece of DNA
  6. 10. Cells that have the potential to become any type of cell
  7. 12. An enzyme that acts like a glue connecting the phosphate backbone of a piece of DNA inserted into a cloning vector
  8. 13. Bacterial scissors or enzymes that recognize specific DNA sequences and cut the DNA at those sites
  9. 15. Cells that have already been programmed to be a certain type of cell
  10. 17. The process of deciding what traits should be expressed in the next generation
  11. 18. Something like a computer chip Thousands of DNA sequences can be spotted on a chip to identify which sequences are present in a sample the determination of the order of nitrogenous bases in DNA
  12. 21. dna sequence and the cloning vector into which it has been inserted
Down
  1. 2. The removal of the nucleus of one cell and insertion into an egg cell where the nucleus has been removed
  2. 6. The use of biotechnology to manipulate an organism's genome
  3. 7. these are cells that have the potential to become any type of cell
  4. 8. a small piece of DNA maintained separately from the chromosome
  5. 11. The insertion of a gene of interest into a souped-up vehicle or vector that allows a single gene to be studied
  6. 14. These cells can be differentiated and then coaxed to become a completely different type of cell
  7. 16. The use of a labeled (fluorescent or radioactive) sequence of RNA or DNA that is complementary to the sequence one wants to detect and localize
  8. 19. A short sequence (usually two to five nucleotides in length) of DNA that is repeated
  9. 20. Specific DNA sequence recognized by a restriction enzyme