Botany

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Across
  1. 3. Area containing the bacterial chromosome
  2. 4. pre-vacuum autoclave ________________ is a type of autoclave.
  3. 7. staining ______________ is used for identification of mycobacterium
  4. 8. Coal-tar derivatives
  5. 10. _____________ refers to the process of attaching cell to a slide
  6. 12. Methylene blue test is a test to check the efficacy of
  7. 14. In endospore staining, ______________ method was heat to push the primary stain.
  8. 20. fission Bacteria are divided by
  9. 22. Acid-fast staining uses ____________ as primary stain
  10. 24. water Kills most vegetative bacteria and viruses immediately
  11. 25. oxide A highly effective chemisterilant
  12. 27. The employment of techniques to achieve microbe-free environment
  13. 29. foils While sterilization process, individual glass articles must be wrapped in
  14. 32. A form of aldehyde used for surface disinfection of rooms
  15. 33. Sugar and gelatine in medium are exposed to free steaming for 20 minutes for three successive days. This process is known as
  16. 34. Diffusion of water across a semi-permeable membrane
  17. 36. Dry heating includes Red heat, flaming, Hot air oven and __________
Down
  1. 1. sterilization Radiation does not generate heat, so it is termed as
  2. 2. Full form of BPL
  3. 5. method Method of preservation of pure culture that results in the death of microbes after sometimes
  4. 6. death time The minimum time required to kill a suspension of organisms at a predetermined temperature in a specified environment
  5. 9. An elongate, hollow appendage used in transfer of DNA to other cell & cell adhesion
  6. 11. A visible mass of microorganisms all originating from a single mother cell
  7. 13. culture method Method used to isolate microorganisms, which are present in relatively small numbers or that have slow growth rate
  8. 15. The material placed onto the medium is called the
  9. 16. plate method Method which involves plating of diluted sample mixed with melted agar-medium
  10. 17. Bath The contaminating bacteria in a _____ can be inactivated by heating in a water bath at 60o C for one hour
  11. 18. It is a state in which the growth of bacteria is suppressed without being killed
  12. 19. rays Sterilized items such as metal instruments and glassware are sterilized with the help of
  13. 21. Efficiency Particle Air Full form of HEPA
  14. 23. stain Stain that colors the body of the cells in capsule staining
  15. 26. Bacteria are ___________ organisms
  16. 28. plate method The method in which a small amount of mixed culture is placed on the tip of an inoculation loop and is streak across the surface of agar-medium
  17. 30. filters Pasteur-Chamberland filter is a type of
  18. 31. wall Gram positive and gram negative bacteria have differences in
  19. 35. gradient By streaking, _____________ is established across the face of the petri plate as bacterial cells are deposited on the agar surface