BOTC Lecture Chapter 10

3. A collection of restriction fragments from a species of interest that has been cloned in bacteria; like a collection of books.
7. DNA that contains information from two different individuals or species.
8. Once you've cut DNA you'll 'paste' it together with this.
9. A small, circular DNA used as a vector in transformation.
12. Denaturing a mixture of DNA or RNA such that renaturation results in a mixture of double-stranded molecules; for probing purposes.
15. The enzyme that makes DNA from an RNA template.
16. DNAs that 'target' a sequence for amplification.
17. A DNA library specifically populated by reverse-transcribed fragments of mRNA.

1. Method of separating/viewing molecules by size and charge in a gel matrix.
2. Production of many identical copies of a DNA sequence.
4. The name of the researcher that developed a widely used DNA sequencing method.
5. Causing a cell to take up DNA that changes its phenotype.
6. in-vitro DNA replication.
10. An organism that contains DNA from another species.
11. DNA or RNA 'Scissors'; invented by bacteria in nature.
13. The name of the researcher that developed a nucleic acid hybridization method for probing a genome.
14. Plural abbreviation for the building blocks used in a PCR.