Chapter 3 - Observing Microorganisms through a Microscope

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Across
  1. 5. Has a series of lenses and uses visible light as a source of illumination.
  2. 6. Attached to the surface of a glass slide.
  3. 7. The group of bacteria that stain red during the gram stain.
  4. 13. A thin film of material, containing the microorganisms, is spread over the surface of the slide.
  5. 14. Used to color parts of microorganisms, such as endospores, flagella, or capsules.
  6. 15. Foreign substance that invokes antibody production.
  7. 17. Calculated by multiplying the objective lens magnification by the ocular lens magnification.
  8. 22. Eyepiece.
  9. 23. Structures of locomotion to small to be seen with a light microscope without staining.
  10. 25. Diagnostic technique in which fluorochromes are attached to antibodies in order to adhere and identify certain pathogenic bacteria.
  11. 29. Natural defense molecules that are produced by humans and many animals in reaction to a foreign substance (antigen).
  12. 30. A finely focused beam of electrons passes through a prepared specimen, providing detailed imagery of the interior of the specimen.
  13. 32. Crystal Violet is this type of stain in the gram stain protocol.
  14. 33. Gelatinous covering contained by some bacteria.
  15. 34. Special resistant, dormant structure formed within a cell that protects a bacterium from adverse environmental conditions.
  16. 35. A finely focused beam of electrons strike the surface of a prepared specimen, providing detailed imagery of the exterior of the specimen.
Down
  1. 1. The measure of the light-bending ability of a medium.
  2. 2. Chromophore is the anion in these dyes.
  3. 3. These types of stains can be used to differentiate between different types of bacteria and different types of bacterial structures.
  4. 4. The light source on a light microscope.
  5. 8. These types of stains have a contrasting color to primary stains like crystal violet.
  6. 9. This type of differential staining technique is used to differentiate gram-positive and gram-negative bacteria.
  7. 10. The group of bacteria that stain purple during the gram stain.
  8. 11. These are the lenses that are the closest to the specimen and can be manipulated by the observer.
  9. 12. Preparing colorless bacteria against a colored background.
  10. 14. Coloring the microorganisms with a dye that emphasizes certain structures.
  11. 16. This type of differential stain technique is used to distinguish between bacteria that have a waxy material in their cell walls.
  12. 18. Chemical is added to the solution to intensify the stain and to increase the affinity of a stain for a biological specimen.
  13. 19. Chromophore is the cation in these dyes.
  14. 20. The ability of substances to absorb short wavelengths of light (ultraviolet) and give off light at a longer wavelength (visible).
  15. 21. The ability of the lenses to distinguish fine detail and structure (also called resolving power).
  16. 24. Microscopy technique in which a beam of electrons are used instead of visible light, since electrons travel in a wavelength much smaller than visible light, this group of microscopes has a much greater resolving power.
  17. 26. The alcohol wash solution during the gram stain protocol is used to remove the crystal violet from the gram negative bacteria.
  18. 27. Contains lenses that direct the light through the specimen.
  19. 28. The degree to which a pathogen can cause disease.
  20. 31. Aqueous or alcohol solution of a single basic dye.