Cloning DNA and Plasmids and Transformation

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Across
  1. 2. often provides the necessary machinery for a gene to be expressed.
  2. 6. region in plasmid that has been engineered to contain recognition sites of a number of restriction endonucleases.
  3. 7. first step in cloning a gene
  4. 8. transforming cells through electrical shock.
  5. 10. number of copies of a particular plasmid found in a bacterial cell.
  6. 11. a fragment of DNA that has been introduced into a foreign cell, resulting in exact copies of the original DNA fragment being made when the foreign cell replicates and divides.
  7. 12. Cell a cell that has been taken up a foreign plasmid or virus whose cellular machinery is being used to express the foreign DNA.
  8. 13. method that can be used to isolate the cells with recombinant DNA.
  9. 18. vehicles by which DNA may be introduced into host cells.
  10. 19. plasmids do not have one of these
Down
  1. 1. a cell that readily takes up foreign DNA.
  2. 3. where the plasmid naturally exists
  3. 4. plasmids enter bacteria through _______ in the membrane.
  4. 5. used to splice a foreign gene into a plasmid.
  5. 9. bacterium with resistance to as many as 4 antibiotics.
  6. 10. classical method of transforming cells.
  7. 14. the relationship between bacteria and plasmids.
  8. 15. small circular pieces of DNA that can exit and enter bacterial cells.
  9. 16. plasmids often carry genes that express proteins able to confer ________ resistance.
  10. 17. introduction of foreign DNA.