Crossword : Engineering an artificiel PPR protein to regulate the expression of a chloroplast gene

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Across
  1. 6. A molecular biology technique that consists in producing a recombinant DNA and using it to transform a host organism
  2. 10. A PentatricoPeptide R… protein (PPR) is a protein binding chloroplast RNA
  3. 11. Important enzyme in the Calvin cycle of the photosynthesis, one sub-unit is encoded by the nuclear genome and the other by the chloroplast genome
  4. 13. A restriction enzyme in the family of nucleases that binds DNA
  5. 14. The model plant in vegetal biology
  6. 16. The word corresponding to the M of the “EMSA” technique
  7. 18. A DNA sequence necessary to the transcription of the downstream coding sequence
  8. 19. Tag use to purify a protein on a nickel column
Down
  1. 1. The RBP protein family name stands for RNA B… Protein
  2. 2. A microorganism that infect plants, causing tumors and opines production, also used in biotechnologies to transfer a T-DNA in another organism
  3. 3. Prokaryote RNA sequence recognized by the ribosome to initiate translation
  4. 4. The herbicide whose resistance gene is the bar gene
  5. 5. The plant-specific organelle
  6. 7. The amino acid whose code is S
  7. 8. The structure in the chloroplast where the photosynthesis occurs
  8. 9. A technic used to transfer protein on a membrane to reveal and identify them
  9. 12. The technique used to transform plant female gametophyte
  10. 15. The reaction made by plants to convert light energy into chemical energy
  11. 17. A big protein composed of two sub-unit responsible of the translation of RNA in protein
  12. 18. Circular DNA of bacteria using in biotechnologies as a vector