Crossword : Engineering an artificiel PPR protein to regulate the expression of a chloroplast gene

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Across
  1. 1. Important enzyme in the Calvin cycle of the photosynthesis, one sub-unit is encoded by the nuclear genome and the other by the chloroplast genome
  2. 3. Circular DNA of bacteria using in biotechnologies as a vector
  3. 6. A restriction enzyme in the family of nucleases that binds DNA
  4. 10. The model plant in vegetal biology
  5. 11. The plant-specific organelle
  6. 12. The RBP protein family name stands for RNA B… Protein
  7. 14. A DNA sequence necessary to the transcription of the downstream coding sequence
  8. 16. & Dalgarno Prokaryote RNA sequence recognized by the ribosome to initiate translation
  9. 18. The structure in the chloroplast where the photosynthesis occurs
  10. 19. The herbicide whose resistance gene is the bar gene
  11. 20. The amino acid whose code is S
Down
  1. 2. A molecular biology technique that consists in producing a recombinant DNA and using it to transform a host organism
  2. 4. A microorganism that infect plants, causing tumors and opines production, also used in biotechnologies to transfer a T-DNA in another organism
  3. 5. A technic used to transfer protein on a membrane to reveal and identify them
  4. 7. A PentatricoPeptide R… protein (PPR) is a protein binding chloroplast RNA
  5. 8. The technique used to transform plant female gametophyte
  6. 9. The reaction made by plants to convert light energy into chemical energy
  7. 13. Tag use to purify a protein on a nickel column
  8. 15. The word corresponding to the M of the “EMSA” technique
  9. 17. A big protein composed of two sub-unit responsible of the translation of RNA in protein