Crossword : Engineering an artificiel PPR protein to regulate the expression of a chloroplast gene

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Across
  1. 2. The amino acid whose code is S
  2. 4. Circular DNA of bacteria using in biotechnologies as a vector
  3. 7. The word corresponding to the M of the “EMSA” technique
  4. 11. enzyme in the Calvin cycle of the photosynthesis, one sub-unit is encoded by the nuclear genome and the other by the chloroplast genome
  5. 12. A molecular biology technique that consists in producing a recombinant DNA and using it to transform a host organism
  6. 16. A technic used to transfer protein on a membrane to reveal and identify them
  7. 17. A microorganism that infect plants, causing tumors and opines production, also used in biotechnologies to transfer a T-DNA in another organism
  8. 18. A PentatricoPeptide R… protein (PPR) is a protein binding chloroplast RNA
  9. 19. Tag use to purify a protein on a nickel column
  10. 20. & Dalgarno Prokaryote RNA sequence recognized by the ribosome to initiate translation
Down
  1. 1. The model plant in vegetal biology
  2. 3. A restriction enzyme in the family of nucleases that binds DNA
  3. 5. The structure in the chloroplast where the photosynthesis occurs
  4. 6. A big protein composed of two sub-unit responsible of the translation of RNA in protein
  5. 8. The herbicide whose resistance gene is the bar gene
  6. 9. The reaction made by plants to convert light energy into chemical energy
  7. 10. The RBP protein family name stands for RNA B… Protein
  8. 13. A DNA sequence necessary to the transcription of the downstream coding sequence
  9. 14. The plant-specific organelle
  10. 15. The technique used to transform plant female gametophyte