CROSSWORD PUZZLE

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Across
  1. 5. can be use to smooth muscle skin.
  2. 6. ak.a Moller’s Fluid.
  3. 9. Defined as the killing, penetration and hardening of tissues.
  4. 10. Recommended for urgent biopsies, for specimens like lungs, brain, eyes, spleen and CNS, considered as the most rapid.
  5. 11. similar to paraplast; melting point of 56-58 deg C.
  6. 14. for delicate tissues.
  7. 16. Clearing agent obtained from a citrus fruit.
  8. 20. can be use to clear smooth muscle of skin.
  9. 22. use for ricketssia and othe bacteria.
  10. 25. determine cause of death.
  11. 28. Removal of calcium ions or lime salts from the organic extracellular matrix, calcified collagen and surrounding.
  12. 29. also known as ETHYLENE GLYCOL MONOETHYL ETHER.
  13. 30. are those fixatives use to preserve parts of the nucleus like nuclear chromatin, chromosome.
  14. 31. cutting off excess paraffin wax to form a four-sided prism.
  15. 34. use only for urgent biopsies.
  16. 36. For infiltrating bones, brain, teeth and whole organ.
  17. 38. Recommended only for frozen section and histochemical and enzyme studies.
  18. 40. routine dehydrating agent, commonly used because it is fast acting and non toxic.
  19. 41. allows the general microscopic study of tissue structures without altering the structural pattern and normal intercellular relationship of tissues.
  20. 45. Need to have auto technicon the automatic tissue processor.
  21. 49. dissolves paraffin.
  22. 52. AKA Collodion
  23. 53. a water soluble wax for enzyme
  24. 56. contains absolute alcohol, glacial HAC and chloroform.
  25. 57. remove clearing agent from tissues.
  26. 58. Most commonly used infiltrating agent because the process is very rapid.
Down
  1. 1. Can be use as ethanol substitute.
  2. 2. fixatives that when used combined with the tissues or absorbed/ absorbed by the tissue takin in by the tissue.
  3. 3. Not absorbed, not takin in by the tissue.
  4. 4. are fixatives use to preserve the chemical component of tissues like enzyme.
  5. 7. also known as Diethylene Dioxide.
  6. 8. routine clearing agent.
  7. 11. Harder than paraffin and requires heavy duty type of microtome.
  8. 12. Embedding for EM.
  9. 13. not commonly use because it is toxic and is suited only for blood and tissue films.
  10. 15. CT mucins & umbilical cord.
  11. 17. most reliable/ most accurate because this method can detect even smallest amt of calcium.
  12. 18. PRESENCE OF THIS WOULD MEAN THAT DECALCIFICATION IS NOT YET COMPLETE.
  13. 19. Corrosive a.k.a. Formol Sublimate.
  14. 20. Recommended for plants and animal micro technique.
  15. 21. product of paraffin with rubber.
  16. 23. recommended for fixation of embryos and pituitary biopsy and endometrial curettings.
  17. 24. are those that preserve parts of the cytoplasm like golgi body, mitochondria and so on
  18. 26. most common chrome osmium acetic acid fixative, excellent for nuclear structures.
  19. 27. May be used for neurochemical substances in brain like acetylcholine.
  20. 28. For infiltrating eye specimen.
  21. 32. classified both as nuclear and histochemical fixative.
  22. 33. - mixture of highly purified paraffin and synthetic plastic polymers.
  23. 35. it is fast acting fixative, regarded as smallest aldehyde fixative.
  24. 37. Carried out using oven the purpose is to keep the wax liquid.
  25. 39. Used for infiltrating eye specimen.
  26. 42. automatic tissue processor that can do fixation until infiltration.
  27. 43. for GIT biopsies & endocrine tissues.
  28. 44. a.k.a. Hely’s Fluid.
  29. 46. For rough tissues (skin, fibroid and decalcified tissues) for nervous tissues,lymph nodes and embryos.
  30. 47. Cheap, easy to prepare and readily available, stable.
  31. 48. are fixative use to preserve parts of the cell either nucleus or cytoplasm.
  32. 50. Recommended for clearing embryos for clearing insects and other very delicate insects and other delicate specimens.
  33. 51. eyes can use for eye specimen, it has a faint odor and low evaporation rate the reason why it can be a good substitute from cedarwood oil.
  34. 54. Use for acid mucopolyssacharide and also for tissue mucin.
  35. 55. Tissues tend to become adulterated.