D1.1

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Across
  1. 2. The original DNA strand used as a pattern for the synthesis of a new strand.
  2. 3. A technique used to identify individuals based on unique patterns in their DNA.
  3. 6. A permanent change in the DNA sequence resulting from a replication error.
  4. 7. A method used to rapidly copy specific DNA sequences.
  5. 8. The first step of PCR where high heat separates the DNA double helix.
  6. 9. Short DNA sequences that bind to a target to provide a starting point for Taq.
  7. 13. A technique used to separate DNA fragments based on their size and charge.
  8. 14. The step in PCR where primers bind to the complementary target sequences.
  9. 15. The building block of DNA consisting of a sugar, phosphate, and nitrogenous base.
  10. 18. The enzyme that unwinds and separates the double helix by breaking hydrogen bonds.
Down
  1. 1. The process of making multiple copies of a specific DNA segment using PCR.
  2. 4. The strong bond formed between the phosphate of one nucleotide and the sugar of the next.
  3. 5. Replication where each new DNA molecule contains one original strand and one new strand.
  4. 10. The enzyme that assembles new DNA strands by linking nucleotides in a 5' to 3' direction.
  5. 11. The type of weak bond that holds the two strands of the DNA helix together.
  6. 12. The enzyme that joins DNA fragments together to create a continuous strand.
  7. 16. A heat-stable enzyme used in PCR that does not denature at high temperatures.
  8. 17. The rule that adenine pairs with thymine and cytosine pairs with guanine.