DNA RECOMBINANT TECHNOLOGY

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Across
  1. 1. Another name for DNA cloning vector
  2. 5. Organism that used to be the host cell
  3. 8. _______________ is the process where bacteria host cell will be cloned
  4. 10. __________ ends show straight cut cutting pattern
  5. 11. The bacteria colony that remains ______ shows that X-gal cannot be hydrolyzed
  6. 13. ______________ will hydrolyzes X-gal
  7. 16. Only bacteria that has the ______________ plasmid will grow in gene cloning
  8. 19. ___________ is the technique to identify clone cells with rDNA
  9. 20. Example of restriction enzyme
  10. 21. Genetically engineered plasmid are re-introduced into the bacterium in the process called ___________
Down
  1. 2. Type of enzyme that used to be modifying enzyme
  2. 3. DNA ligase will bind the two gene fragments and produced ________
  3. 4. Gene of interest and plasmid will have the same ___________ ends
  4. 6. Bacteria without ______ gene, unable to hydrolyze lactose
  5. 7. Source of plasmid and human gene will be ______ at the first step in gene cloning
  6. 9. __________ the plasmid and gene of interest by using the same restriction enzyme
  7. 12. Gene code for insulin (_____ DNA)
  8. 14. Another name for restriction enzyme
  9. 15. Type of cutting pattern (_______ ends)
  10. 17. DNA cloning vector
  11. 18. Gene of interest will be _________ into plasmid