Electrophoresis

2. proteins if globular, then surface area is?
4. Gradient formed in isoelectric focusing to separate protein
5. poor medium causes
9. The direction of fluid is towards?
12. Reducing agent that breaks disulfide bonds in proteins
16. Migration of charged particles in medium requires?
18. The gel layer responsible for separating protein by size
20. the typical shape of double stranded DNA molecules, which affects how they move through gel pores
22. The overall speed of protein migration in native PAGE depends on size and this property
23. Stain used to visualize proteins after native PAGE

1. Dye used in electrophoresis to monitor sample migration
3. suppose there are 3 types of DNA- ssDNA ,dsDNA and ds plasmid and you are to carry out electrophoresis, which of these will have faster electrophoretic mobility?
4. a buffer not to be used for proteins
6. A method of PAGE that denatures proteins, unlike native PAGE
7. The gel commonly used in PAGE to separate proteins
8. The Fragment of DNA with a known sequence used as a standard in electrophoresis
9. Used to form loading wells in a gel, removed after the gel has set
10. Proteins can be separated using electrophoresis based on?
11. the parameter that determines the degree of ionization of organic compounds
13. The buffer system often used to maintain protein structure during native PAGE
14. Type of material exhibiting electroendosmosis
15. this fluorescent dye binds between the stacked base pairs of DNA, making it visible under ultraviolet light
17. Factor influencing electroendosmosis flow?
19. This enzyme is commonly used to cleave DNA into fragments for electrophoresis
21. Gel component with lower acrylamide concentration for protein separation