ELISA Crossword

Edit Answers
12345678910111213
Across
  1. 2. The animal that the detection antibody was produced in
  2. 4. The “IS” of ELISA stands for
  3. 5. The ELISA format described as, “a primary antibody conjugated to an enzyme that binds to an antigen on a plate”
  4. 7. The method of dilution you used to prepare your IgG standards
  5. 10. The term that describes antibodies that are identical, produced by a clone of a single antibody producing cell
  6. 11. The colour generated by the substrate, TMB in the ELISA
  7. 12. The total number of wells of the 96-well plate you are using in your experiment today
  8. 13. The step of the ELISA that prevents binding of protein to parts of the plate not occupied by the capture antibody
Down
  1. 1. In this experiment, your secondary antibody is conjugated to Horseradish Peroxidase (HRP); what is HRP?
  2. 3. What is measured at 450 nm as the outcome of the ELISA?
  3. 6. Running a sample in __________ is a standard scientific practice to improve the reliability of results by allowing for error detection and reducing variability.
  4. 8. Columns one, two and three are used for this ____________ curve
  5. 9. This column is used for the patient samples