Fixation, Dehydration, Clearing and Infiltration

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Across
  1. 2. a method of testing the completeness of decalcification by pricking the tissue.
  2. 4. a clearing agent obtained from citrus.
  3. 6. an infiltration method that is rarely used and recommended only for frozen sections.
  4. 7. a positive result of this method is cloudiness which means decalcification is not yet complete.
  5. 10. a celloidin method that produces hard tissue blocks and allows cutting thinner sections.
  6. 12. a type of celloidin method used for infiltrating bones, brain, teeth and whole organs.
  7. 13. a method of paraffin impregnation that is the most rapid because it generates heat.
  8. 14. is the most commonly used infiltrating agent.
  9. 17. a dehydrating agent that dissolve paraffin.
  10. 19. a routine clearing agent.
  11. 22. the color mercury deposits when mercuric chloride is used
  12. 24. cutting off excess paraffin wax to form a four-sided prism.
  13. 27. it is used for acid mucopolysaccharide and for tissue mucin.
  14. 28. ethanol substitute and xylene substitute.
  15. 29. fixative that is recommended for preservation of enzymes.
  16. 30. fixative used specifically to preserve parts of cytoplasm.
  17. 32. a clearing agent that is a substitute for xylene and benzene
  18. 34. a substitute for paraffin wax that is used for infiltrating eye specimens.
  19. 35. similar to a paraplast with a melting point of 56-58 C.
  20. 39. a dehydrating agent recommended for animals, plants and microincineration techniques.
  21. 40. fixative used specifically to preserve parts of nucleus.
  22. 41. most important and crucial step in tissue processing.
  23. 42. it is placing infiltrated tissue in a mold containing the embedding medium.
  24. 43. fixative used for preserving bone marrow.
  25. 44. a 100% of this alcohol is for fixing and drying blood smears and bone marrow tissues.
  26. 49. a mixture of highly purified paraffin and synthetic plastic polymers.
  27. 51. effect of hypotonic solution to cell.
  28. 53. a water-soluble wax for enzyme histochemistry.
  29. 54. fixative used to preserve parts of the cell.
  30. 55. fixative used to preserve chemical component of tissue.
Down
  1. 1. method that immersion of specimen in chemical fixatives is performed.
  2. 2. it imparts yellow color if used as a tissue fixative.
  3. 3. other name for celloidin infiltration method.
  4. 5. a routine dehydrating agent.
  5. 6. it is recommended for histochemistry & Electron Microscopy.
  6. 8. used for routine tissue processing.
  7. 9. a clearing agent for eyes specimen.
  8. 11. a wax with a much lower melting point 46-48°C.
  9. 15. a fixative and dehydrating agent that easily evaporates.
  10. 16. recommended for fixing spleen, liver and connective tissue fibers & nuclei.
  11. 18. most rapid method of decalcification.
  12. 20. most reliable and most accurate method of testing the completeness of decalcification.
  13. 21. also known as diethylene dioxide.
  14. 23. a reagent used to preserve tissue.
  15. 25. a clearing agent that is toxic to liver.
  16. 26. an embedding used for extremely hard tissues like epoxy and polyester.
  17. 31. the removal of dehydrating agent from tissues.
  18. 33. the smallest aldehyde fixative.
  19. 34. a clearing agent that is carcinogenic ang highly flammable.
  20. 36. a celloidin infiltration method that is used for infiltrating eye specimens.
  21. 37. a technique used for fixing neurochemical substances in brain.
  22. 38. a disposable mold that is economical and easy to prepare.
  23. 39. a method of testing the completeness of decalcification carried out by adding calcium carbonate.
  24. 45. product of paraffin with rubber.
  25. 46. involves removal of clearing agent from tissues.
  26. 47. most rapid method of paraffin wax impregnation.
  27. 48. a 95% of this alcohol is for touch preparation.
  28. 50. removal of intercellular and extracellular water from tissues after fixation.
  29. 52. a method of paraffin impregnation that is carried out using an oven.