Fixation, Dehydration, Clearing and Infiltration

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Across
  1. 5. removal of intercellular and extracellular water from tissues after fixation.
  2. 7. most rapid method of paraffin wax impregnation.
  3. 10. a clearing agent that is toxic to liver.
  4. 12. most important and crucial step in tissue processing.
  5. 13. a clearing agent that is carcinogenic ang highly flammable.
  6. 15. the color mercury deposits when mercuric chloride is used
  7. 16. a reagent used to preserve tissue.
  8. 18. similar to a paraplast with a melting point of 56-58 C.
  9. 19. a 100% of this alcohol is for fixing and drying blood smears and bone marrow tissues.
  10. 20. other name for celloidin infiltration method.
  11. 23. the smallest aldehyde fixative.
  12. 25. it is used for acid mucopolysaccharide and for tissue mucin.
  13. 27. cutting off excess paraffin wax to form a four-sided prism.
  14. 29. a dehydrating agent that dissolve paraffin.
  15. 30. fixative used for preserving bone marrow.
  16. 31. a method of paraffin impregnation that is carried out using an oven.
  17. 33. a water-soluble wax for enzyme histochemistry.
  18. 34. fixative used to preserve parts of the cell.
  19. 37. a substitute for paraffin wax that is used for infiltrating eye specimens.
  20. 39. is the most commonly used infiltrating agent.
  21. 42. a physical fixation method not usually carried out in histophatology
  22. 45. a routine clearing agent.
  23. 47. fixative that is recommended for preservation of enzymes.
  24. 49. product of paraffin with rubber.
  25. 51. a wax with a much lower melting point 46-48°C.
  26. 52. most rapid method of decalcification.
  27. 54. effect of hypotonic solution to cell.
  28. 55. fixative used specifically to preserve parts of nucleus.
  29. 57. a routine dehydrating agent.
  30. 59. a technique used for fixing neurochemical substances in brain.
  31. 60. a 95% of this alcohol is for touch preparation.
Down
  1. 1. a type of celloidin method used for infiltrating bones, brain, teeth and whole organs.
  2. 2. ethanol substitute and xylene substitute.
  3. 3. a clearing agent for eyes specimen.
  4. 4. used for routine tissue processing.
  5. 6. involves removal of clearing agent from tissues.
  6. 8. the removal of dehydrating agent from tissues.
  7. 9. it imparts yellow color if used as a tissue fixative.
  8. 11. a clearing agent obtained from citrus.
  9. 14. terpineol is used with this kind of specimen.
  10. 17. also known as diethylene dioxide.
  11. 21. a disposable mold that is economical and easy to prepare.
  12. 22. fixative used specifically to preserve parts of cytoplasm.
  13. 24. a fixative and dehydrating agent that easily evaporates.
  14. 26. a method of paraffin impregnation that is the most rapid because it generates heat.
  15. 28. a method of testing the completeness of decalcification carried out by adding calcium carbonate.
  16. 32. a specimen that must undergo intravascular perfusion
  17. 35. it is placing infiltrated tissue in a mold containing the embedding medium.
  18. 36. a clearing agent that is a substitute for xylene and benzene
  19. 38. most reliable and most accurate method of testing the completeness of decalcification.
  20. 40. a type of fixative as to mechanism of action when used it is absorbed by the tissue
  21. 41. method that immersion of specimen in chemical fixatives is performed.
  22. 43. presence of these are due to incomplete incomplete washing of tissues.
  23. 44. a mixture of highly purified paraffin and synthetic plastic polymers.
  24. 46. a method of testing the completeness of decalcification by pricking the tissue.
  25. 48. an infiltration method that is rarely used and recommended only for frozen sections.
  26. 50. a dehydrating agent recommended for animals, plants and microincineration techniques.
  27. 53. an embedding used for extremely hard tissues like epoxy and polyester.
  28. 56. a positive result of this method is cloudiness which means decalcification is not yet complete.
  29. 58. a celloidin infiltration method that is used for infiltrating eye specimens.