flow cytometry

1. - Fluorescent protein commonly used in flow cytometry
5. - Method used to correct spectral overlap between fluorophores
8. - Commonly used DNA stain in flow cytometry
10. - Cellular property measured by forward scatter (FSC)
14. - Scientist who first developed the fluorescence-activated cell sorter (FACS)
16. - Fluorescent molecule used to label cells
18. analysis - Technique to detect minimal residual disease in hematological malignancies
19. - Laser commonly used for red-excited fluorophores (e.g., APC, Alexa 647)

2. - Software commonly used for flow cytometry data analysis
3. - Fluorophore commonly used in clinical flow cytometry for CD45 staining
4. - Scientist who pioneered the use of monoclonal antibodies in flow cytometry
6. - Technique that combines flow cytometry with single-cell RNA sequencing
7. - Antibody target for distinguishing live from dead cells in fixable viability dyes
9. or negative - Type of charge applied to droplets in electrostatic sorting
11. - Process of sorting cells based on fluorescence or size
12. - Multiplexed technique that allows detection of over 40 markers using metal-conjugated antibodies
13. - Fluorescent probe used to measure intracellular calcium levels
15. - Algorithm used to cluster single-cell flow cytometry data
16. - Regulatory agency that sets guidelines for clinical flow cytometry assays
17. - Company that commercialized the first FACS instrument