Genetic Engineering

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Across
  1. 3. When a gene is inactivated through genetic engineering, it is a gene ______.
  2. 5. We put antibiotic resistance markers on plasmids so that we can use this process to keep only cells that retain the plasmid.
  3. 8. A _______ fusion is a method used to study gene expression by putting a fluorescent protein reporter downstream of a native promoter
  4. 12. These type of enzymes cut DNA at a specific sequence
  5. 14. An animal for which only some of its somatic cells carry a genetic modification
  6. 15. This type of insertion occurs when your vector is inserted at a random position in the genome
  7. 18. This type of genetic material is made by using reverse transcriptase on mRNA
  8. 19. This phase of a PCR is when primers bind to the DNA template through complementary base pairing.
  9. 20. This enzyme is used to form the bond between a cut vector and an insert.
Down
  1. 1. This type of eukaryotic stem cell can be cultured in a dish and genetically modified, then used to produce a full-grown animal.
  2. 2. This word is used to describe pieces of DNA that have been cut to have a 5’ or 3’ overhang
  3. 4. ________ recombination is used to insert a vector at a targeted location in the genome
  4. 6. This component of a cloning vector ensures that the host cell replicates the vector.
  5. 7. This technique adds a fluorescent protein in-frame to the end of the protein you’re trying to study
  6. 9. This type of DNA is formed by laboratory methods bringing together DNA from different sources.
  7. 10. This occurs when the cell repairs a double-strand break by randomly ligating together two loose ends of DNA
  8. 11. This type of organism contains genetic material from another organism.
  9. 13. The gene _______ technique alters the sequence of a gene in the genome but does not inactivate it.
  10. 14. This is the set of experimental methods used to replicate recombinant DNA molecules in a bacterial or eukaryotic host
  11. 16. The ________ transcriptase enzyme makes a DNA copy from an mRNA template
  12. 17. This type of cloning vector is a small circular DNA molecule typically found in prokaryotes