Genetics Lab 3 Puzzle

Edit Answers
123456789101112131415161718192021222324252627282930313233343536373839
Across
  1. 4. Single-stranded DNA sequences that attach to template DNA.
  2. 10. The PCR step after denaturation where primers bind to DNA.
  3. 14. 5'-TACCAT-3' Could this be a restriction site?
  4. 15. The optimal temperature for restriction enzymes, in celsius.
  5. 17. Temperature of PCR denaturation (In words and celsius)
  6. 21. ______ DNA fragments are found near the top of the gel.
  7. 23. The 'am' of BamHI represents which name of the original bacteria?
  8. 24. What we use to make the gel for electrophoresis. Creates a meshwork.
  9. 25. ______ DNA pieces move further through a gel.
  10. 26. BamHI makes a _______ cut into DNA.
  11. 29. The 'H' of BamHI represents which name of the original bacteria?
  12. 30. A ______ consists of known DNA sizes to help us estimate the size of DNA fragments during electrophoresis.
  13. 31. The primer that attaches to the template DNA strand.
  14. 32. What we use to visualize DNA under UV light. Embeds itself into DNA.
  15. 33. Latin phrase that means an experiment performed without a living organism.
  16. 34. First step of PCR
  17. 37. The 'I' of HhaI means that this was the _____ restriction enzyme to be isolated from Haemophilus haemolyticus.
  18. 38. DNA cut with BamHI has an ______ on the 5' end.
  19. 39. The extension step of PCR is usually performed around this temperature.
Down
  1. 1. Sticky-ended cuts can form ________ with complementary DNA sequences.
  2. 2. DNA moves towards the ______ end of the gel during electrophoresis.
  3. 3. 5'-CGATCG-3' Could this be a restriction site?
  4. 4. A circular form of DNA.
  5. 5. A restriction site located in a non-coding region that varies per individual. Can be used to determine if a child belongs to a parent.
  6. 6. How many copies of DNA would you expect after 10 cycles of PCR? (In words, no and)
  7. 7. The electrophoresis running buffer contains _____ to help produce a current.
  8. 8. HaeIII makes a _________ cut into DNA.
  9. 9. If you digest a DNA plasmid with EcoRI and run the product on a gel, if five bands appear, how many EcoRI sites are present in the plasmid?
  10. 11. If you digest a linear DNA fragment with EcoRI and run the product on a gel, if one band appears, how many EcoRI sites are present in the DNA fragment?
  11. 12. Restriction enzymes recognize _____ sequences.
  12. 13. Nucleotides that are involved in PCR.
  13. 16. Genomic DNA appears as a _____ after restriction digest and gel electrophoresis.
  14. 18. 5'-ACCTGT-3' Could this be a restriction site?
  15. 19. The 'H' in HaeIII represents which name of the original bacteria?
  16. 20. The first restriction enzyme isolated from E. coli
  17. 22. The primer that attaches to the complementary DNA strand.
  18. 27. The final PCR step where DNA polymerase extends the DNA.
  19. 28. Metal ions that stabilize a PCR reaction.
  20. 34. The enzyme in PCR.
  21. 35. DNA has a ______ charge.
  22. 36. The annealing step during PCR is usually around this temperature.