Genetics terminology

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Across
  1. 2. the process of adding genes to or removing genes from a plasmid (two words)
  2. 3. the second step of PCR that cools to ~60 degrees to allow primers to dock to DNA templates
  3. 4. regions of loosely condensed DNA where genes are available for expression
  4. 5. the DNA backbone wraps around these positively charged protein during condensation
  5. 10. the enzyme that can use mRNA as a template to synthesize DNA (two words)
  6. 12. a gene whose mRNA encodes for multiple proteins
  7. 15. an experiment that quantifies mRNA levels in a sample to correlate to protein expression levels
  8. 16. the region upstream of the gene that is added to the mRNA during processing (contains a number)
Down
  1. 1. the third step of PCR that warms to ~72 degrees to allow the polymerase to function
  2. 2. a gene whose mRNA encodes for only one protein
  3. 6. the portions of mRNA that are removed during splicing of eukaryotic genes
  4. 7. regions of highly condensed DNA
  5. 8. the region upstream of the gene that is removed during mRNA processing of eukaryotic genes (contains a number)
  6. 9. the first step of PCR that heats to ~95 degrees to separate DNA strands
  7. 11. the portions of mRNA that are retained during splicing of eukaryotic genes
  8. 13. the region downstream of the gene that is removed during mRNA processing of eukaryotic genes (contains a number)
  9. 14. the region downstream of the gene that is added to the mRNA during processing (two words)